puc118 cloning vector  (TaKaRa)

Journal: Cell Reports Methods

Article Title: De-erosion of X chromosome dosage compensation by the editing of XIST regulatory regions restores the differentiation potential in hPSCs

doi: 10.1016/j.crmeth.2022.100352

Figure Lengend Snippet:

Article Snippet: PUC118 cloning vector , Takara Bio Inc. , Cat# 3324.

Techniques: Recombinant, Plasmid Preparation, Lysis, Nick Translation, DNA Methylation Assay, Clone Assay, Expressing, Knock-Out, Software

Journal: Chemosphere

Article Title: Organoarsenicals inhibit bacterial peptidoglycan biosynthesis by targeting the essential enzyme MurA

doi: 10.1016/j.chemosphere.2020.126911

Figure Lengend Snippet: Fig. 1. DNA isolated from an S. putrefaciens 200 genomic library confers MAs(III) resistance. Overnight cultures of E. coli TOP10 carrying either, pUC118-Clone 1 (-), pUC19-ArsI (B), pTrcHis2A-ArsP (;), or plasmid pUC118 (C) were adjusted to a starting absorbance at 600 nm of 0.05 in 2x ST media supplemented with 0.2% glucose containing the indicated concentrations of MAs(III). Growth was estimated from A600

Article Snippet: The resulting DNA fragment mixture was ligated overnight at 16 C into HindIII-digested cloning vector pUC118 using LONG ligase (TaKaRa, Kusatsu, Japan).

Techniques: Isolation, Plasmid Preparation

Journal: Chemosphere

Article Title: Organoarsenicals inhibit bacterial peptidoglycan biosynthesis by targeting the essential enzyme MurA

doi: 10.1016/j.chemosphere.2020.126911

Figure Lengend Snippet: Fig. 2. Expression of murA confers resistance to MAs(III) in vivo. Overnight cultures of E. coli TOP10 carrying either pUC118-Clone 1, which contains wild-type S. putrefaciens 200 murA and bolA (C), vector carrying a murA tyrosine-to-stop codon mutant (D), vector carrying a bolA tyrosine-to-stop codon mutation (;), or parental plasmid pUC118 (B) were adjusted to a starting A600 nm of 0.05 in 2x ST media supplemented with 0.2% glucose containing the indicated concentrations of MAs(III). Growth was estimated from the A600 nm after 5 h incubation at 30 C. Data are the mean ± SE (n ¼ 3).

Article Snippet: The resulting DNA fragment mixture was ligated overnight at 16 C into HindIII-digested cloning vector pUC118 using LONG ligase (TaKaRa, Kusatsu, Japan).

Techniques: Expressing, In Vivo, Plasmid Preparation, Mutagenesis, Incubation